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Nat Rev Genet. 2015 Mar;16(3):159-71. doi: 10.1038/nrg3900. Epub 2015 Feb 10.

Chromatin regulation at the frontier of synthetic biology.

Author information

1
1] Department of Biomedical Engineering and Center of Synthetic Biology, Boston University, Boston, Massachusetts 02215, USA. [2] Institute for Medical Engineering &Science, Synthetic Biology Center and Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts 02139, USA.
2
1] Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA. [2] Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
3
1] Department of Biomedical Engineering and Center of Synthetic Biology, Boston University, Boston, Massachusetts 02215, USA. [2] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.
4
1] Institute for Medical Engineering &Science, Synthetic Biology Center and Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts 02139, USA. [2] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA. [3] Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.

Abstract

As synthetic biology approaches are extended to diverse applications throughout medicine, biotechnology and basic biological research, there is an increasing need to engineer yeast, plant and mammalian cells. Eukaryotic genomes are regulated by the diverse biochemical and biophysical states of chromatin, which brings distinct challenges, as well as opportunities, over applications in bacteria. Recent synthetic approaches, including 'epigenome editing', have allowed the direct and functional dissection of many aspects of physiological chromatin regulation. These studies lay the foundation for biomedical and biotechnological engineering applications that could take advantage of the unique combinatorial and spatiotemporal layers of chromatin regulation to create synthetic systems of unprecedented sophistication.

PMID:
25668787
PMCID:
PMC4846386
DOI:
10.1038/nrg3900
[Indexed for MEDLINE]
Free PMC Article

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