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Ann Plast Surg. 2015 Mar;74(3):371-5. doi: 10.1097/SAP.0000000000000439.

Implanted scaffold-free prevascularized constructs promote tissue repair.

Author information

1
From the *Department of Regenerative Medicine and Cell Biology, †Department of Surgery, Medical University of South Carolina, Charleston, SC.

Abstract

To evaluate the anastomotic potential of prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells, tissue constructs were implanted into athymic mice and immune-competent rats. Analysis of xenografts placed into hind limb muscle defects showed vascular anastomotic activity by 3 days after implantation and persisting for 2 weeks. Integration of the implanted prevascular tissue constructs with the host circulatory system was evident from presence of red blood cells in the implant as early as 3 days after implantation. Additionally, analysis of 3-day xenografts in the rat model showed activation of skeletal muscle satellite cells based on Pax-7 and MyoD expressions. We conclude that prevascular tissue constructs generated from scaffold-free self-assembly of human endothelial and fibroblast cells are a promising tool to provide both vascular supply and satellite cell activation toward the resolution of skeletal muscle injury.

PMID:
25668498
PMCID:
PMC4324532
DOI:
10.1097/SAP.0000000000000439
[Indexed for MEDLINE]
Free PMC Article

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