Format

Send to

Choose Destination
J Invest Dermatol. 2015 Jun;135(6):1510-1520. doi: 10.1038/jid.2015.41. Epub 2015 Feb 10.

Distribution of bioactive lipid mediators in human skin.

Author information

1
Faculty of Medical and Human Sciences, Manchester Pharmacy School, The University of Manchester, Manchester, UK.
2
Dermatology Centre, Institute of Inflammation and Repair, Faculty of Medical and Human Sciences, The University of Manchester, Manchester, UK.
3
School of Pharmacy and Centre for Skin Sciences, School of Life Sciences, University of Bradford, Bradford, UK.
4
Safety and Environmental Assurance Centre, Unilever, Sharnbrook, UK.
5
Faculty of Medical and Human Sciences, Manchester Pharmacy School, The University of Manchester, Manchester, UK. Electronic address: anna.nicolaou@manchester.ac.uk.

Abstract

The skin produces bioactive lipids that participate in physiological and pathological states, including homeostasis, induction, propagation, and resolution of inflammation. However, comprehension of the cutaneous lipid complement, and contribution to differing roles of the epidermal and dermal compartments, remains incomplete. We assessed the profiles of eicosanoids, endocannabinoids, N-acyl ethanolamides, and sphingolipids, in human dermis, epidermis, and suction blister fluid. We identified 18 prostanoids, 12 hydroxy-fatty acids, 9 endocannabinoids and N-acyl ethanolamides, and 21 non-hydroxylated ceramides and sphingoid bases, several demonstrating significantly different expression in the tissues assayed. The array of dermal and epidermal fatty acids was reflected in the lipid mediators produced, whereas similarities between lipid profiles in blister fluid and epidermis indicated a primarily epidermal origin of suction blister fluid. Supplementation with omega-3 fatty acids ex vivo showed that their action is mediated through perturbation of existing species and formation of other anti-inflammatory lipids. These findings demonstrate the diversity of lipid mediators involved in maintaining tissue homeostasis in resting skin and hint at their contribution to signaling, cross-support, and functions of different skin compartments. Profiling lipid mediators in biopsies and suction blister fluid can support studies investigating cutaneous inflammatory responses, dietary manipulation, and skin diseases lacking biomarkers and therapeutic targets.

PMID:
25668241
DOI:
10.1038/jid.2015.41
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center