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ISME J. 2015 Sep;9(9):2108-11. doi: 10.1038/ismej.2015.14. Epub 2015 Feb 10.

Chlorophyll f-driven photosynthesis in a cavernous cyanobacterium.

Author information

1
1] Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark [2] Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark [3] Section for Biomolecular Sciences, Department of Biology, University of Copenhagen, Copenhagen N, Denmark.
2
Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
3
Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
4
1] Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark [2] Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.

Abstract

Chlorophyll (Chl) f is the most recently discovered chlorophyll and has only been found in cyanobacteria from wet environments. Although its structure and biophysical properties are resolved, the importance of Chl f as an accessory pigment in photosynthesis remains unresolved. We found Chl f in a cyanobacterium enriched from a cavernous environment and report the first example of Chl f-supported oxygenic photosynthesis in cyanobacteria from such habitats. Pigment extraction, hyperspectral microscopy and transmission electron microscopy demonstrated the presence of Chl a and f in unicellular cyanobacteria found in enrichment cultures. Amplicon sequencing indicated that all oxygenic phototrophs were related to KC1, a Chl f-containing cyanobacterium previously isolated from an aquatic environment. Microsensor measurements on aggregates demonstrated oxygenic photosynthesis at 742 nm and less efficient photosynthesis under 768- and 777-nm light probably because of diminished overlap with the absorption spectrum of Chl f and other far-red absorbing pigments. Our findings suggest the importance of Chl f-containing cyanobacteria in terrestrial habitats.

PMID:
25668158
PMCID:
PMC4542031
DOI:
10.1038/ismej.2015.14
[Indexed for MEDLINE]
Free PMC Article

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