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J Gen Virol. 2015 Jun;96(Pt 6):1478-83. doi: 10.1099/vir.0.000074. Epub 2015 Feb 9.

Synthetic long peptide booster immunization in rhesus macaques primed with replication-competent NYVAC-C-KC induces a balanced CD4/CD8 T-cell and antibody response against the conserved regions of HIV-1.

Author information

1
Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands mooij@bprc.nl.
2
Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.
3
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
4
University of Regensburg, Franz-Josef-Strauss Allee 11, D93053 Regensburg, Germany.
5
Centro Nacional de Biotecnologia, CSIC, Madrid, Spain.
6
Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland Swiss Vaccine Research Institute, Lausanne, Switzerland.
7
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
8
Arizona State University, Tempe, Arizona, USA.
9
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands ISA pharmaceuticals, J.H. Oortweg 19-21, 2333 CH Leiden, The Netherlands.

Abstract

The Thai trial (RV144) indicates that a prime-boost vaccine combination that induces both T-cell and antibody responses may be desirable for an effective HIV vaccine. We have previously shown that immunization with synthetic long peptides (SLP), covering the conserved parts of SIV, induced strong CD4 T-cell and antibody responses, but only modest CD8 T-cell responses. To generate a more balanced CD4/CD8 T-cell and antibody response, this study evaluated a pox-vector prime/SLP boost strategy in rhesus macaques. Priming with a replication-competent NYVAC, encoding HIV-1 clade C gag, pol and nef, induced modest IFNγ T-cell immune responses, predominantly directed against HIV-1 Gag. Booster immunization with SLP, covering the conserved parts of HIV-1 Gag, Pol and Env, resulted in a more than 10-fold increase in IFNγ ELISpot responses in four of six animals, which were predominantly HIV-1 Pol-specific. The animals showed a balanced polyfunctional CD4 and CD8 T-cell response and high Ab titres.

PMID:
25667320
DOI:
10.1099/vir.0.000074
[Indexed for MEDLINE]

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