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FEBS Lett. 2015 Mar 12;589(6):718-25. doi: 10.1016/j.febslet.2015.01.039. Epub 2015 Feb 7.

Redox-dependent translocation of the heat shock transcription factor AtHSFA8 from the cytosol to the nucleus in Arabidopsis thaliana.

Author information

1
Biochemistry and Physiology of Plants, Bielefeld University, 33615 Bielefeld, Germany.
2
Biochemistry and Physiology of Plants, Bielefeld University, 33615 Bielefeld, Germany. Electronic address: karl-josef.dietz@uni-bielefeld.de.

Abstract

The hypothesis is tested that some heat stress transcription factors (HSFs) are activated after formation of inter- or intramolecular disulfide bonds. Based on in silico analyses we identified conserved cysteinyl residues in AtHSFA8 that might function as redox sensors in plants. AtHSFA8 represents a redox-sensitive transcription factor since upon treatment of protoplasts with H2O2 YFP-labeled HSFA8 was translocated to the nucleus in a time-dependent manner. Site-directed mutagenesis of the conserved residues Cys24 and Cys269 blocked translocation of HSFA8 to the nucleus. The findings concur with a model where HSFA8 functions as redox sensing transcription factor within the stress-responsive transcriptional network.

KEYWORDS:

AtHSFA8; Cytoplasmic-nuclear shuttling; Fluorescence resonance energy transfer; Heat stress transcription factor; Reactive oxygen species; Redox-regulation

PMID:
25666709
DOI:
10.1016/j.febslet.2015.01.039
[Indexed for MEDLINE]
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