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Nat Chem Biol. 2015 Mar;11(3):198-200. doi: 10.1038/nchembio.1753. Epub 2015 Feb 9.

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.

Author information

1
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
2
1] Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA. [2] Center for Genomic and Computational Biology, Duke University, Durham, North Carolina, USA. [3] Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA.

Abstract

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

PMID:
25664691
PMCID:
PMC4412021
DOI:
10.1038/nchembio.1753
[Indexed for MEDLINE]
Free PMC Article

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