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Int Immunopharmacol. 2015 Mar;25(1):218-28. doi: 10.1016/j.intimp.2015.01.027. Epub 2015 Feb 7.

Beneficial effects of neomangiferin on high fat diet-induced nonalcoholic fatty liver disease in rats.

Author information

1
Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University at Shenyang, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China.
2
Ordos Central Hospital, Ordos School of Clinical Medicines, Inner Mongolia Medical University, Ordos 017000, China.
3
Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University at Shenyang, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China. Electronic address: 893927534@qq.com.

Abstract

This study was carried out to determine the effect and mechanism of action of neomangiferin (NG) on high-fat diet-induced nonalcoholic fatty liver disease (NAFLD) in rats. NAFLD rats were randomly assigned into several groups of equal number. NG (50, 25mg/kg·day(-1) BW) and lipanthyl (PT, 5mg/kg·day(-1) BW) were given to the NAFLD rats, respectively. In the study, serum lipids, metabolic rate, liver fat, liver lipids and histology were examined. To further investigate the molecular mechanism of the effect of NG on NAFLD, expression levels of mRNA and protein for peroxisome proliferator-activated receptor α (PPARα), fatty acid transport protein 2 (FATP2), long-chain-fatty-acid - CoA ligase 1 (ACSL1) and carnitine palmitoyltransferase 1a (CPT1a) in the liver were determined by Real Time-PCR and western blot analysis, respectively. NG administration significantly reduced the final body weight, liver fat accumulation, and serum triglyceride (TG), total cholesterol (TC) concentrations, low-density lipoprotein cholesterol (LDL-C), glucose (GLU) levels, and hepatic TG, TC, malondialdehyde (MDA) levels, but increased serum high-density lipoprotein cholesterol (HDL-C) and hepatic superoxide dismutase (SOD) levels. NG upregulated the mRNA and protein expression of PPARα and CPT1a, but downregulated the mRNA and protein expression of FATP2 and ACSL1 in the liver. These results suggested that NG can regulate NAFLD partly by modulating the expression levels of genes involved in FFA uptake and lipid oxidation.

KEYWORDS:

Fatty acid transport protein 2; High-fat diet; Neomangiferin; Nonalcoholic fatty liver; Peroxisome proliferator-activated receptor α

PMID:
25661699
DOI:
10.1016/j.intimp.2015.01.027
[Indexed for MEDLINE]

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