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J Biosci Bioeng. 2015 Aug;120(2):155-60. doi: 10.1016/j.jbiosc.2014.12.011. Epub 2015 Feb 7.

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains.

Author information

1
Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, Republic of Korea. Electronic address: gopalakrishnanchml@gmail.com.
2
Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
3
Department of Environmental Engineering and Science, Feng Chia University, Taichung 40724, Taiwan.
4
Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, Republic of Korea.

Abstract

In this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. Firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°C, 30 min) sludge and liquid (LB-grown) cultures of Escherichia coli XL1-Blue/Enterobacter cloacae DSM 16657 strains for bioaugmentation purposes. The results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in the form of inoculum. Furthermore, experiments were carried out in order to reveal whether the increment occurred either due to the efficient contribution of the facultative anaerobic microorganisms or the culture ingredients (in particular yeast extract and tryptone) supplied when the bacterial suspensions (LB media-based inocula) were mixed with the sludge. The outcome of these tests was that both the applied nitrogen sources and the bacteria (E. coli) could individually enhance hydrogen formation. Nevertheless, the highest increase took place when they were used together. Finally, the optimal initial wastewater concentration was determined as 5 g/L.

KEYWORDS:

Beverage wastewater; Bioaugmentation; Enterobacter cloacae DSM 16657; Escherichia coli XL1-Blue; External nitrogen source

PMID:
25661265
DOI:
10.1016/j.jbiosc.2014.12.011
[Indexed for MEDLINE]

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