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Anal Biochem. 2015 May 1;476:29-35. doi: 10.1016/j.ab.2015.01.023. Epub 2015 Feb 4.

Sandwich enzyme-linked immunosorbent assay for the quantification of human serum albumin fragment 408-423 in bodily fluids.

Author information

1
Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; International Graduate School in Molecular Medicine Ulm, Ulm University, 89081 Ulm, Germany.
2
Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.
3
ELISA-Entwicklung, 53359 Rheinbach, Germany.
4
Core Unit Mass Spectrometry and Proteomics, Ulm University, 89081 Ulm, Germany.
5
Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Ulm Peptide Pharmaceuticals, Ulm University, 89081 Ulm, Germany.
6
Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Ulm Peptide Pharmaceuticals, Ulm University, 89081 Ulm, Germany. Electronic address: jan.muench@uni-ulm.de.

Abstract

Urinary levels of human serum albumin (hSA) fragment 408-423 have been proposed to represent an early marker for graft-versus-host disease (GvHD) and chronic kidney diseases. Here, we developed an enzyme-linked immunosorbent assay (ELISA) for the quantification of hSA(408-423). The sandwich ELISA has a detection limit of 0.5ng/ml and is highly specific for hSA(408-423) because it does not cross-react with other albumin fragments or the full-length precursor. This ELISA allows rapid and convenient quantification of hSA(408-423) in bodily fluids, further clarifying the prognostic and diagnostic value of this peptide in GvHD, kidney disease, and other disorders.

KEYWORDS:

Biomarker; ELISA; Graft-versus-host disease; Human serum albumin; Kidney disease; Peptide

PMID:
25660532
DOI:
10.1016/j.ab.2015.01.023
[Indexed for MEDLINE]

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