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Cell Cycle. 2015;14(3):333-41. doi: 10.4161/15384101.2014.980647.

Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication.

Author information

1
a Department of Biology ; University of York ; York , UK.

Abstract

The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.

KEYWORDS:

DNA replication; MCM, Minichromosome maintenance; MCM2-7, nuclear matrix; NM, Nuclear matrix; cell cycle; initiation; minichromosome maintenance complex

PMID:
25659032
PMCID:
PMC4615129
DOI:
10.4161/15384101.2014.980647
[Indexed for MEDLINE]
Free PMC Article

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