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Proteomics Clin Appl. 2015 Apr;9(3-4):289-94. doi: 10.1002/prca.201400202. Epub 2015 Mar 21.

Is label-free LC-MS/MS ready for biomarker discovery?

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Department of Immunotechnology, Lund University, Lund, Sweden.


Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label-free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future.


Label-free; Mass spectrometry

[Indexed for MEDLINE]

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