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Biomed Res Int. 2015;2015:580240. doi: 10.1155/2015/580240. Epub 2015 Jan 14.

Identification and characterization of a serious multidrug resistant Stenotrophomonas maltophilia strain in China.

Author information

1
Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China ; The Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China ; Department of Respiratory Diseases, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.
2
Department of Critical Care Medicine, Affiliated Hospital of Academy of Military Medical Science, Beijing 100071, China.
3
Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
4
The Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
5
Beijing Institute of Biotechnology, Beijing 100071, China.
6
Department of Respiratory Diseases, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China.

Abstract

An S. maltophilia strain named WJ66 was isolated from a patient; WJ66 showed resistance to more antibiotics than the other S. maltophilia strains. This bacteraemia is resistant to sulphonamides, or fluoroquinolones, while the representative strain of S. maltophilia, K279a, is sensitive to both. To explore drug resistance determinants of this strain, the draft genome sequence of WJ66 was determined and compared to other S. maltophilia sequences. Genome sequencing and genome-wide evolutionary analysis revealed that WJ66 was highly homologous with the strain K279a, but strain WJ66 contained additional antibiotic resistance genes. Further analysis confirmed that strain WJ66 contained an amino acid substitution (Q83L) in fluoroquinolone target GyrA and carried a class 1 integron, with an aadA2 gene in the resistance gene cassette. Homology analysis from the pathogen-host interaction database showed that strain WJ66 lacks raxST and raxA, which is consistent with K279a. Comparative genomic analyses revealed that subtle nucleotide differences contribute to various significant phenotypes in close genetic relationship strains.

PMID:
25654114
PMCID:
PMC4310304
DOI:
10.1155/2015/580240
[Indexed for MEDLINE]
Free PMC Article

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