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PLoS One. 2015 Feb 3;10(2):e0117067. doi: 10.1371/journal.pone.0117067. eCollection 2015.

Arabidopsis HFR1 is a potential nuclear substrate regulated by the Xanthomonas type III effector XopD(Xcc8004).

Author information

1
Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan; Ph.D. Program in Microbial Genomics, National ChungHsing University and Academia Sinica, Taipei, Taiwan.
2
Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan.
3
Department of Plant Pathology, National ChungHsing University, Taichung, Taiwan.
4
Institute of Biochemistry, National ChungHsing University, Taichung, Taiwan; Ph.D. Program in Microbial Genomics, National ChungHsing University and Academia Sinica, Taipei, Taiwan; Institute of Biotechnology, National ChungHsing University, Taichung, Taiwan; NCHU-UCD Plant and Food Biotechnology Center, National ChungHsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National ChungHsing University, Taichung, Taiwan.

Abstract

XopDXcc8004, a type III effector of Xanthomonas campestris pv. campestris (Xcc) 8004, is considered a shorter version of the XopD, which lacks the N-terminal domain. To understand the functions of XopDXcc8004, in planta, a transgenic approach combined with inducible promoter to analyze the effects of XopDXcc8004 in Arabidopsis was done. Here, the expression of XopDXcc8004, in Arabidopsis elicited the accumulation of host defense-response genes. These molecular changes were dependent on salicylic acid and correlated with lesion-mimic phenotypes observed in XVE::XopDXcc8004 transgenic plants. Moreover, XopDXcc8004 was able to desumoylate HFR1, a basic helix-loop-helix transcription factor involved in photomorphogenesis, through SUMO protease activity. Interestingly, the hfr1-201 mutant increased the expression of host defense-response genes and displayed a resistance phenotype to Xcc8004. These data suggest that HFR1 is involved in plant innate immunity and is potentially regulated by XopDXcc8004.

PMID:
25647296
PMCID:
PMC4315394
DOI:
10.1371/journal.pone.0117067
[Indexed for MEDLINE]
Free PMC Article

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