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Methods Mol Biol. 2016;1373:63-8. doi: 10.1007/7651_2014_185.

Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method.

Author information

1
Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA.
2
Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA. jbose@kumc.edu.

Abstract

The ability to move DNA between Staphylococcus strains is essential for the genetic manipulation of this bacterium. Often in the Staphylococci, this is accomplished through transduction using generalized transducing phage and can be performed in different ways and therefore the presence of two transduction procedures in this book. The following protocol is a relatively easy-to-perform, broth-based procedure that we have used extensively to move both plasmids and chromosomal fragments between strains of Staphylococcus aureus.

KEYWORDS:

Bacteriophage; Transduction; phi11; phi80α

PMID:
25646607
DOI:
10.1007/7651_2014_185
[Indexed for MEDLINE]

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