An UPLC-MS/MS method for determination of solasonine in rat plasma and its application of a pharmacokinetic and bioavailability study

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Mar 15:985:1-5. doi: 10.1016/j.jchromb.2015.01.017. Epub 2015 Jan 23.

Abstract

Solasonine, a known glycoalkaloid, is a potential anti-cancer agent. In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of solasonine in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an ACQUITY HSS T3 (100×2.1mm, 1.8μm) column with a gradient mobile phase consisting of 0.1% formic acid and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1-500ng/mL (R(2)>0.994) with a low limit of detection (0.1ng/mL) and lower limit of quantification (0.5ng/mL). The extraction recovery was in the range of 92.4-94.9% for solasonine and 91.9% for dendrobine (internal standard, IS). The intra- and inter-day precision was below 9.8% and accuracy was from 86.0% to 94.3%. No notable matrix effect and astaticism was observed for solasonine. The method has been successfully applied to a pharmacokinetic and bioavailability study of solasonine in rats for the first time, which provides the basis for the further development and application of solasonine.

Keywords: Bioavailability; Pharmacokinetics; Solasonine; UPLC-MS/MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Availability
  • Chromatography, High Pressure Liquid / methods*
  • Limit of Detection
  • Linear Models
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Solanaceous Alkaloids / blood*
  • Solanaceous Alkaloids / chemistry
  • Solanaceous Alkaloids / pharmacokinetics
  • Tandem Mass Spectrometry / methods*

Substances

  • Solanaceous Alkaloids
  • alpha-solamargine