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J Microbiol Methods. 2015 Apr;111:21-3. doi: 10.1016/j.mimet.2015.01.024. Epub 2015 Jan 30.

MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay.

Author information

1
Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
2
Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
3
Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada. Electronic address: garrett.westmacott@phac-aspc.gc.ca.

Abstract

MALDI-TOF MS detection of carbapenemase-activity in Gram-negative bacteria was compared against the Carba-NP assay. MALDI-TOF MS detected activity from 99% of the strains, from all types of carbapenemase (200/202), while Carba-NP assays detected activity from 85% (45/53) of the tested isolates and could not consistently identify OXA- or GES carbapenemase activity.

KEYWORDS:

Acinetobacter; Carbapenemase detection; Enterobacteriaceae; MALDI-TOF; Pseudomonas

PMID:
25644285
DOI:
10.1016/j.mimet.2015.01.024
[Indexed for MEDLINE]

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