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EMBO Rep. 2015 Mar;16(3):387-95. doi: 10.15252/embr.201439757. Epub 2015 Feb 2.

Single muscle fiber proteomics reveals unexpected mitochondrial specialization.

Author information

1
Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany Department of Biomedical Sciences, University of Padova, Padua, Italy mmurgia@biochem.mpg.de stefano.schiaffino@unipd.it mmann@biochem.mpg.de.
2
Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
3
Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
4
Department of Biomedical Sciences, University of Padova, Padua, Italy.
5
Venetian Institute of Molecular Medicine, Padua, Italy.
6
Venetian Institute of Molecular Medicine, Padua, Italy mmurgia@biochem.mpg.de stefano.schiaffino@unipd.it mmann@biochem.mpg.de.
7
Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark mmurgia@biochem.mpg.de stefano.schiaffino@unipd.it mmann@biochem.mpg.de.

Abstract

Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

KEYWORDS:

exercise; metabolism; mitochondria; muscle fibers; single cell

PMID:
25643707
PMCID:
PMC4364878
DOI:
10.15252/embr.201439757
[Indexed for MEDLINE]
Free PMC Article

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