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Nat Methods. 2015 Mar;12(3):199-202, 4 p following 202. doi: 10.1038/nmeth.3281. Epub 2015 Feb 2.

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

Author information

1
Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
2
Cavendish Laboratory, University of Cambridge, Cambridge, UK.
3
1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Department of Physics, Creighton University, Omaha, Nebraska, USA.
4
Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
5
Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
6
1] Max Planck Institute for the Physics of Complex Systems, Dresden, Germany. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
7
1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Cavendish Laboratory, University of Cambridge, Cambridge, UK.

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

PMID:
25643151
DOI:
10.1038/nmeth.3281
[Indexed for MEDLINE]

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