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Nat Methods. 2015 Mar;12(3):227-9, 1 p following 229. doi: 10.1038/nmeth.3257. Epub 2015 Feb 2.

A sensor for quantification of macromolecular crowding in living cells.

Author information

1
1] Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands. [2] Zernike Institute for Advanced Materials, University of Groningen, Groningen, the Netherlands.
2
Department of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.

Abstract

Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.

PMID:
25643150
DOI:
10.1038/nmeth.3257
[Indexed for MEDLINE]

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