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Nat Methods. 2015 Mar;12(3):207-10, 2 p following 210. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2.

Reporters for sensitive and quantitative measurement of auxin response.

Author information

1
Laboratory of Biochemistry, Wageningen University, Wageningen, the Netherlands.
2
Laboratoire de Reproduction et Développement des Plantes, CNRS, Institut National de la Recherche Agronomique, Ecole Normale Superieure Lyon, Lyon, France.

Abstract

The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.

PMID:
25643149
PMCID:
PMC4344836
DOI:
10.1038/nmeth.3279
[Indexed for MEDLINE]
Free PMC Article

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