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Cancer Lett. 2015 Apr 10;359(2):307-13. doi: 10.1016/j.canlet.2015.01.036. Epub 2015 Jan 29.

Toxoplasma gondii infection in cancer patients: prevalence, risk factors, genotypes and association with clinical diagnosis.

Author information

1
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin, China.
2
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China.
3
Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130000, Jilin, China.
4
Weihaiwei People's Hospital, Weihai 264200, Shandong, China.
5
Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, Shandong, China.
6
Wendeng Municipal Hospital, Weihai 264200, Shandong, China.
7
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin, China.
8
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Jilin, China. Electronic address: xingquanzhu1@hotmail.com.

Abstract

Prevalence of human infection with Toxoplasma gondii has been increasing in China due to the increasing number of cats. However, little is known of the epidemiology of T. gondii infection in different cancer patient groups. Thus, a case-control study of 900 cancer patients and 900 controls was conducted to detect anti-T. gondii antibodies by ELISA in China. Genomic DNA was extracted from the diseased tissues of 510 patients and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genetically characterized using multi-locus PCR-RFLP. The prevalence of anti-T. gondii IgG in cancer patients (35.56%) was significantly higher than that in controls (17.44%). The highest T. gondii seroprevalence was detected in lung cancer patients (60.94%), followed by cervical cancer patients (50%), brain cancer patients (42.31%) and endometrial cancer patients (41.67%). Exposure with soil and consumption of raw/undercooked meat were significantly associated with T. gondii infection in cancer patients. Three T. gondii genotypes (ToxoDB#9, ToxoDB#10 and Type I variant) were identified. In conclusion, T. gondii infection is a severe problem in cancer patients and it is imperative that improved integrated measures should be conducted to prevent and control T. gondii infection in cancer patients.

KEYWORDS:

Cancer patients; Genotype; Prevalence; Risk factors; Toxoplasma gondii

PMID:
25641340
DOI:
10.1016/j.canlet.2015.01.036
[Indexed for MEDLINE]

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