Format

Send to

Choose Destination
J Physiol. 2015 Jun 15;593(12):2627-34. doi: 10.1113/jphysiol.2014.287714. Epub 2015 Mar 13.

Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology.

Author information

1
Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
2
Department of Anesthesiology, Pharmacology, and Therapeutics, University of British Columbia, Vancouver, BC, Canada.
3
Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA.

Abstract

Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.

PMID:
25640301
PMCID:
PMC4500348
DOI:
10.1113/jphysiol.2014.287714
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center