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J Mycol Med. 2015 Jun;25(2):e89-93. doi: 10.1016/j.mycmed.2014.10.023. Epub 2014 Nov 13.

In vitro activity of carvacrol and thymol combined with antifungals or antibacterials against Pythium insidiosum.

Author information

1
Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, RS, Brazil.
2
Programa de Pós-Graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Departamento de Microbiologia e Parasitologia, Av. Roraima n° 1000, Prédio 20, sala 4139, CEP 97105-900 Santa Maria, RS, Brazil.
3
Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil.
4
Programa de Pós-Graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Departamento de Microbiologia e Parasitologia, Av. Roraima n° 1000, Prédio 20, sala 4139, CEP 97105-900 Santa Maria, RS, Brazil. Electronic address: janio.santurio@gmail.com.

Abstract

We describe the in vitro activities of the combinations of carvacrol and thymol with antibiotics (azithromycin, clarithromycin, minocycline and tigecycline) and antifungal agents (amphotericin B, caspofungin, itraconazole and terbinafine) against 23 isolates of the oomycete Pythium insidiosum. The assays were based on the M38-A2 technique and checkerboard microdilution. Based on the mean FICI values, the main synergies observed were combinations of carvacrol+itraconazole and thymol+itraconazole (96%), thymol+clarithromycin (92%), carvacrol+clarithromycin (88%), thymol+minocycline (84%), carvacrol+minocycline (80%), carvacrol+azithromycin (76%), thymol+azithromycin (68%), carvacrol+tigecycline (64%) and thymol+tigecycline (60%). In conclusion, we found that combinations of carvacrol or thymol with these antimicrobial agents might provide effective alternative treatments for cutaneous pythiosis due to their synergistic interactions. Future in vivo experiments are needed to elucidate the safety and therapeutic potential of these combinations.

KEYWORDS:

Activités synergiques anti-Pythium; Carvacrol; Combination of drugs; Pythiose; Pythiosis; Susceptibility test; Technique M38-A2; Test en damier; Thymol

PMID:
25639921
DOI:
10.1016/j.mycmed.2014.10.023
[Indexed for MEDLINE]

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