Quantitative assessment of handedness is required in various clinical and research settings in psychology, neuroscience, and medicine. In the present study we tested the reliability and validity of a Japanese version of the FLANDERS handedness questionnaire, which was a new measure of skilled hand preference originally reported by Nicholls, Thomas, Loetscher, and Grimshaw (2013). Participants (N=431) completed three types of handedness questionnaires: the FLANDERS handedness questionnaire, Edinburgh Handedness Inventory, and H · N handedness test. Factor analysis revealed that the Japanese version of FLANDERS handedness questionnaire had a single-factor structure and high internal consistency. This questionnaire also posssed high test-retest reliability and criterion-referenced validity. These results indicate that the Japanese version of the FLANDERS handedness questionnaire is a valid and useful measure of skilled hand preference for Japanese participants.