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Meat Sci. 2015 May;103:83-9. doi: 10.1016/j.meatsci.2015.01.005. Epub 2015 Jan 15.

Metabolic profiling by (1)H NMR of ground beef irradiated at different irradiation doses.

Author information

1
Dipartimento di Scienze degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy. Electronic address: emanuela.zanardi@unipr.it.
2
Dipartimento di Scienze degli Alimenti, Università degli Studi di Parma, Via del Taglio 10, 43126 Parma, Italy.
3
Faculty of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, Keppel Street, WC1E 7HT London, United Kingdom.
4
Dipartimento di Energia, Politecnico di Milano, Via Lambruschini 4, 20156 Milano, Italy.

Abstract

This work describes a metabolic profiling study of non-irradiated and irradiated beef (at 2.5, 4.5 and 8 kGy) using (1)H NMR and chemometrics. The assignment of all major NMR signals of the aqueous/methanolic extracts was performed. A comprehensive multivariate data analysis proved the ability to distinguish between the irradiated and non-irradiated beef. Classification trees revealed that three metabolites (glycerol, lactic acid esters and tyramine or a p-substituted phenolic compound) are important biomarkers for classification of the irradiated and non-irradiated beef samples. Overall, the achieved metabolomic results show that the changes in the metabolic profile of meat provide a valuable insight to be used in detecting irradiated beef. The use of the NMR-based approach simplifies sample preparation and decrease the time required for analysis, compared to available official analytical procedures.

KEYWORDS:

Beef; Classification trees; Irradiation; Metabolomics; NMR; Principal component analysis

PMID:
25637742
DOI:
10.1016/j.meatsci.2015.01.005
[Indexed for MEDLINE]

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