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Curr Opin Biotechnol. 2015 Aug;34:162-70. doi: 10.1016/j.copbio.2015.01.005. Epub 2015 Jan 28.

Proteomics beyond large-scale protein expression analysis.

Author information

1
Institute of Biochemistry, Department of Biology, ETH Zurich, Otto-Stern-Weg 3, CH-8093 Zurich, Switzerland.
2
Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
3
Institute of Biochemistry, Department of Biology, ETH Zurich, Otto-Stern-Weg 3, CH-8093 Zurich, Switzerland. Electronic address: paola.picotti@bc.biol.ethz.ch.

Abstract

Proteomics is commonly referred to as the application of high-throughput approaches to protein expression analysis. Typical results of proteomics studies are inventories of the protein content of a sample or lists of differentially expressed proteins across multiple conditions. Recently, however, an explosion of novel proteomics workflows has significantly expanded proteomics beyond the analysis of protein expression. Targeted proteomics methods, for example, enable the analysis of the fine dynamics of protein systems, such as a specific pathway or a network of interacting proteins, and the determination of protein complex stoichiometries. Structural proteomics tools allow extraction of restraints for structural modeling and identification of structurally altered proteins on a proteome-wide scale. Other variations of the proteomic workflow can be applied to the large-scale analysis of protein activity, location, degradation and turnover. These exciting developments provide new tools for multi-level 'omics' analysis and for the modeling of biological networks in the context of systems biology studies.

PMID:
25636126
DOI:
10.1016/j.copbio.2015.01.005
[Indexed for MEDLINE]

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