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Nat Commun. 2015 Jan 30;6:5724. doi: 10.1038/ncomms6724.

De novo design of an RNA tile that self-assembles into a homo-octameric nanoprism.

Author information

1
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
2
Markey Center for Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
3
The Institute of Respiratory Diseases, Xinqiao Hospital, Chongqing 400037, China.

Abstract

Rational, de novo design of RNA nanostructures can potentially integrate a wide array of structural and functional diversities. Such nanostructures have great promises in biomedical applications. Despite impressive progress in this field, all RNA building blocks (or tiles) reported so far are not geometrically well defined. They are generally flexible and can only assemble into a mixture of complexes with different sizes. To achieve defined structures, multiple tiles with different sequences are needed. In this study, we design an RNA tile that can homo-oligomerize into a uniform RNA nanostructure. The designed RNA nanostructure is characterized by gel electrophoresis, atomic force microscopy and cryogenic electron microscopy imaging. We believe that development along this line would help RNA nanotechnology to reach the structural control that is currently associated with DNA nanotechnology.

PMID:
25635537
DOI:
10.1038/ncomms6724
[Indexed for MEDLINE]

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