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J Mol Cell Biol. 2015 Feb;7(1):12-22. doi: 10.1093/jmcb/mjv006. Epub 2015 Jan 29.

miRNA-181 regulates embryo implantation in mice through targeting leukemia inhibitory factor.

Author information

1
CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China.
2
Department of Cell Biology, Nanjing Medical University, Nanjing 210029, China.
3
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
4
CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei 230027, China qshi@ustc.edu.cn meiyide@ustc.edu.cn wumian@ustc.edu.cn.

Abstract

Embryo implantation is a crucial step in mammalian reproduction. However, little is known regarding the physiological roles of microRNAs in the regulation of embryo implantation. Here we show that a minimum uterine expression of miR-181 is essential for the onset of embryo implantation. Both transient and prolonged transgenic expression of miR-181 led to impaired implantation, which can be rescued by exogenous administration of leukemia inhibitory factor (LIF). Mechanistically, miR-181 is able to directly target LIF and downregulate LIF expression, thereby inhibiting embryo implantation. We also show that miR-181 expression is regulated by the transcriptional factor Emx2, and the Emx2-miR-181 axis plays an important role in regulating embryo implantation. Taken together, these results reveal a novel function of miR-181 in embryo implantation through the regulation of LIF, and also indicate a potential link between miR-181 dysregulation and human embryo implantation defects.

KEYWORDS:

Emx2; LIF; embryo implantation; miR-181

PMID:
25635127
DOI:
10.1093/jmcb/mjv006
[Indexed for MEDLINE]

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