Format

Send to

Choose Destination
J Neurophysiol. 2015 Apr 1;113(7):2713-20. doi: 10.1152/jn.00543.2014. Epub 2015 Jan 28.

High-speed dynamic-clamp interface.

Author information

1
Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York;
2
Department of Computer Science and Engineering, University at Buffalo, State University of New York, Buffalo, New York; and.
3
Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany.
4
Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York; mx@buffalo.edu.

Abstract

The dynamic-clamp technique is highly useful for mimicking synaptic or voltage-gated conductances. However, its use remains rare in part because there are few systems, and they can be expensive and difficult for less-experienced programmers to implement. Furthermore, some conductances (such as sodium channels) can be quite rapid or may have complex voltage sensitivity, so high speeds are necessary. To address these issues, we have developed a new interface that uses a common personal computer platform with National Instruments data acquisition and WaveMetrics IGOR to provide a simple user interface. This dynamic clamp implements leak and linear synaptic conductances as well as a voltage-dependent synaptic conductance and kinetic channel conductances based on Hodgkin-Huxley or Markov models. The speed of the system can be assayed using a testing mode, and currently speeds of >100 kHz (10 μs per cycle) are achievable with short latency and little jitter.

KEYWORDS:

Markov model; WaveMetrics IGOR

PMID:
25632075
PMCID:
PMC4416557
DOI:
10.1152/jn.00543.2014
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center