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Methods Mol Biol. 2015;1264:219-29. doi: 10.1007/978-1-4939-2257-4_20.

In vivo assessment of mitochondrial oxygen consumption.

Author information

1
Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC - University Medical Center Rotterdam, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.

Abstract

The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.

PMID:
25631017
DOI:
10.1007/978-1-4939-2257-4_20
[Indexed for MEDLINE]

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