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Methods Mol Biol. 2015;1264:77-88. doi: 10.1007/978-1-4939-2257-4_8.

A high-throughput next-generation sequencing assay for the mitochondrial genome.

Author information

1
ARUP Laboratories, ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, 84108, USA, shale.dames@aruplab.com.

Abstract

Next-generation sequencing (NGS) is an effective method for mitochondrial genome (mtDNA) sequencing and heteroplasmy detection. The following protocol describes an mtDNA enrichment method up to library preparation and sequencing on Illumina NGS platforms. A short command line alignment script is available for download via FTP.

PMID:
25631005
DOI:
10.1007/978-1-4939-2257-4_8
[Indexed for MEDLINE]

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