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Reproduction. 2015 Apr;149(4):347-55. doi: 10.1530/REP-14-0594. Epub 2015 Jan 27.

High revivability of vitrified-warmed bovine mature oocytes after recovery culture with α-tocopherol.

Author information

1
Graduate School of Science and Technology Faculty of Textile Science and Technology Shinshu University, Nagano 386-8567, Japan National Institute for Physiological Sciences Aichi 444-8787, Japan.
2
Graduate School of Science and Technology Faculty of Textile Science and Technology Shinshu University, Nagano 386-8567, Japan National Institute for Physiological Sciences Aichi 444-8787, Japan Graduate School of Science and Technology Faculty of Textile Science and Technology Shinshu University, Nagano 386-8567, Japan National Institute for Physiological Sciences Aichi 444-8787, Japan.
3
Graduate School of Science and Technology Faculty of Textile Science and Technology Shinshu University, Nagano 386-8567, Japan National Institute for Physiological Sciences Aichi 444-8787, Japan Graduate School of Science and Technology Faculty of Textile Science and Technology Shinshu University, Nagano 386-8567, Japan National Institute for Physiological Sciences Aichi 444-8787, Japan shochi@shinshu-u.ac.jp.

Abstract

The objective of this study was to investigate whether developmental competence of vitrified-warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either l-ascorbic acid or α-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to α-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1-36.3% vs 19.2-25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between α-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in α-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with α-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that α-tocopherol treatment of vitrified-warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.

PMID:
25628440
DOI:
10.1530/REP-14-0594
[Indexed for MEDLINE]

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