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Bioresour Technol. 2015 Apr;181:47-53. doi: 10.1016/j.biortech.2014.12.110. Epub 2015 Jan 9.

Biosynthesis, characterization and enzymatic transesterification of single cell oil of Mucor circinelloides--a sustainable pathway for biofuel production.

Author information

1
Engineering School of Lorena, University of São Paulo, 12602-810 Lorena, São Paulo, Brazil.
2
Engineering School of Lorena, University of São Paulo, 12602-810 Lorena, São Paulo, Brazil. Electronic address: heizir@dequi.eel.usp.br.

Abstract

The filamentous fungus Mucor circinelloides URM 4182 was tested to determine its ability to produce single-cell oil suitable for obtaining biodiesel. Cell growth and lipid accumulation were investigated in a medium containing glucose as the main carbon source. A microwave-assisted ethanol extraction technique (microwave power ⩽200 W, 50-60 °C) was established and applied to lipid extraction from the fungal hyphae to obtain high lipid concentration (44%wt) of the dry biomass, which was considerably higher than the quantity obtained by classical solvent methods. The lipid profile showed a considerable amount of oleic acid (39.3%wt), palmitic acid (22.2%wt) and γ-linoleic acid (10.8%wt). Biodiesel was produced by transesterification of the single-cell oil with ethanol using a immobilized lipase from Candida antarctica (Novozym® 435) as the catalyst. (1)H NMR and HPLC analyses confirmed conversion of 93% of the single-cell oil from M. circinelloides into ethyl esters (FAEE).

KEYWORDS:

Biodiesel; Lipid; Microwave-assisted extraction; Mucor circinelloides; Single-cell oil

PMID:
25625466
DOI:
10.1016/j.biortech.2014.12.110
[Indexed for MEDLINE]
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