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Bioconjug Chem. 2015 Feb 18;26(2):304-15. doi: 10.1021/bc5005735. Epub 2015 Feb 5.

Fluorophore:dendrimer ratio impacts cellular uptake and intracellular fluorescence lifetime.

Author information

1
Department of Chemistry, ‡Department of Biomedical Engineering, and §Department of Physics, University of Michigan , Ann Arbor, Michigan 48109, United States.

Abstract

G5-NH2-TAMRAn (n = 1-4, 5+, and 1.5(avg)) were prepared with n = 1-4 as a precise dye:dendrimer ratio, 5+ as a mixture of dendrimers with 5 or more dye per dendrimer, and 1.5(avg) as a Poisson distribution of dye:dendrimer ratios with a mean of 1.5 dye per dendrimer. The absorption intensity increased sublinearly with n whereas the fluorescence emission and lifetime decreased with an increasing number of dyes per dendrimer. Flow cytometry was employed to quantify uptake into HEK293A cells. Dendrimers with 2-4 dyes were found to have greater uptake than dendrimer with a single dye. Fluorescence lifetime imaging microscopy (FLIM) showed that the different dye:dendrimer ratio alone was sufficient to change the fluorescence lifetime of the material observed inside cells. We also observed that the lifetime of G5-NH2-TAMRA5+ increased when present in the cell as compared to solution. However, cells treated with G5-NH2-TAMRA1.5(avg) did not exhibit the high lifetime components present in G5-NH2-TAMRA1 and G5-NH2-TAMRA5+. In general, the effects of the dye:dendrimer ratio on fluorescence lifetime were of similar magnitude to environmentally induced lifetime shifts.

PMID:
25625297
PMCID:
PMC4636191
DOI:
10.1021/bc5005735
[Indexed for MEDLINE]
Free PMC Article

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