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Food Chem. 2015 Jun 1;176:167-74. doi: 10.1016/j.foodchem.2014.12.061. Epub 2014 Dec 23.

Responses of peripheral blood mononucleated cells from non-celiac gluten sensitive patients to various cereal sources.

Author information

1
Department of Biological, Geological and Environmental Sciences, University of Bologna, Bologna, Italy.
2
Gastroenterology Unit, Spedali Civili and University, Brescia, Italy.
3
Department of Biological, Geological and Environmental Sciences, University of Bologna, Bologna, Italy. Electronic address: enzo.spisni@unibo.it.
4
Department of Agricultural Sciences, University of Bologna, Bologna, Italy.
5
Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy.
6
Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy.
7
Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.

Abstract

Non-celiac gluten sensitivity (NCGS) is still an undefined syndrome whose triggering mechanisms remain unsettled. This study aimed to clarify how cultured peripheral blood mononucleated cells (PBMC) obtained from NCGS patients responded to contact with wheat proteins. Results demonstrated that wheat protein induced an overactivation of the proinflammatory chemokine CXCL10 in PBMC from NCGS patients, and that the overactivation level depends on the cereal source from which proteins are obtained. CXCL10 is able to decrease the transepithelial resistance of monolayers of normal colonocytes (NCM 460) by diminishing the mRNA expression of cadherin-1 (CDH1) and tight junction protein 2 (TJP2), two primary components of the tight junction strands. Thus, CXCL10 overactivation is one of the mechanisms triggered by wheat proteins in PBMC obtained from NCGS patients. This mechanism is activated to a greater extent by proteins from modern with respect to those extracted from ancient wheat genotypes.

KEYWORDS:

Ancient grains; CXCL10; Non celiac gluten sensitivity; Wheat proteins

PMID:
25624220
DOI:
10.1016/j.foodchem.2014.12.061
[Indexed for MEDLINE]
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