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Oncol Lett. 2015 Feb;9(2):645-650. Epub 2014 Nov 28.

microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2.

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Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Department of Oncology, 421 Hospital of the People's Liberation Army, Guangzhou, Guangdong 510318, P.R. China.
Department of Emergency, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Department of Gastroenterology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.


microRNAs (miRNAs/miRs) are a conserved class of endogenous, short non-coding RNAs that post-transcriptionally regulate the expression of genes involved in diverse cellular processes. miR-214 has been reported to be associated with several cancers, including human colon cancer. However, the function of miR-214 in colon cancer development is poorly understood. In the current study, miR-214 was demonstrated to be downregulated in colon cancer tissues compared with healthy colon tissues. Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis. ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214. A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly. In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.


ADP-ribosylation factor-like protein 2; apoptosis; human colon cancer; miR-214; proliferation

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