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Nat Photonics. 2014;8:627-634.

High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues.

Author information

1
Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
2
Semiconductor and Dimensional Metrology Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
3
Department of Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.
4
Department of Cellular and Molecular Medicine, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.

Abstract

An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm-1 to 3500 cm-1) with high resolution (< 10 cm-1). It strongly and efficiently stimulates Raman transitions within the typically weak "fingerprint" region using intrapulse 3-colour excitation, and utilizes the nonresonant background (NRB) to heterodyne amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumours and the surrounding healthy brain matter.

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