Format

Send to

Choose Destination
Vet J. 2015 Mar;203(3):339-41. doi: 10.1016/j.tvjl.2014.12.023. Epub 2014 Dec 29.

Epigenetic mechanisms contribute to enhanced expression of immune response genes in the liver of cows after experimentally induced Escherichia coli mastitis.

Author information

1
Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
2
Clinic for Ruminants, Ludwig-Maximilians-University, Munich, Germany.
3
Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
4
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xzshen@njau.edu.cn.
5
Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany. Electronic address: seyfert@fbn-dummerstorf.de.

Abstract

Endotoxins, such as lipopolysaccharide (LPS), are released during infection with Gram-negative bacteria, which can result in excessive activation of toll-like receptor (TLR) signalling. The aim of the present study was to investigate whether epigenetic mechanisms are involved in controlling the onset and progression of the systemic inflammatory response. Using chromatin accessibility by real-time (CHART) PCR to assess livers from cows with experimentally induced Escherichia coli mastitis, this study demonstrated that the chromatin at the site of the promoters of the genes encoding TLR2, TLR4, lipopolysaccharide binding protein (LBP) and haptoglobin (HP) was opened up 24 h after infection, accompanied by enhanced mRNA expression by these genes. Such modulation did not occur in the same samples for the αS1-casein promoter, which served as a negative control. Demethylation of the TLR4 promoter accompanied opening up of chromatin. These data suggest that modulation of epigenetic factors might offer a novel approach to treating adverse systemic reactions elicited in cows with E. coli mastitis.

KEYWORDS:

Bovine; Chromatin remodelling; DNA-methylation; Liver; Systemic inflammatory response

PMID:
25618856
DOI:
10.1016/j.tvjl.2014.12.023
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center