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Atherosclerosis. 2015 Mar;239(1):203-8. doi: 10.1016/j.atherosclerosis.2015.01.016. Epub 2015 Jan 20.

Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography--animal and human studies.

Author information

1
Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.
2
Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, USA.
3
Division of Nephrology, Mayo Clinic, Rochester, MN, USA.
4
Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA.
5
Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA. Electronic address: Lerman.Amir@mayo.edu.

Abstract

OBJECTIVES:

This study sought to evaluate adventitial vasa vasorum (VV) in vivo with novel imaging technique of optical coherence tomography (OCT).

METHODS:

To verify OCT methods for quantification of VV, we first studied 2 swine carotid arteries in a model of focal angiogenesis by autologous blood injection, and compared microchannel volume (MCV) by OCT and VV by m-CT, and counts of those. In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1 mm from the lumen-intima border. After manually tracing microchannel areas and the boundaries of lumen-intima and media-adventitial in all slices, we reconstructed 3D images. Moreover, we performed with OCT imaging in 8 recipients referred for evaluation of cardiac allograft vasculopathy at 1 year after heart transplantation. MCV and plaque volume (PV) were assessed with 3D images in each 10-mm-segment.

RESULTS:

In the animal study, among the 16 corresponding 1-mm-segments, there were significant correlations of count and volume between both the modalities (count r(2) = 0.80, P < 0.01; volume r(2) = 0.50, P < 0.01) and a good agreement with a systemic bias toward underestimation with m-CT. In the human study, there was a significant positive correlation between MCV and PV (segment number = 24, r(2) = 0.63, P < 0.01).

CONCLUSION:

Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV in vivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.

KEYWORDS:

Cardiac allograft vasculopathy; Microchannel; Microvessel; Optical coherence tomography

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