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J Surg Res. 2015 May 1;195(1):311-4. doi: 10.1016/j.jss.2014.12.039. Epub 2014 Dec 23.

Ex vivo heart perfusion after cardiocirculatory death; a porcine model.

Author information

1
Department of Cardiothoracic Transplantation and Mechanical Circulatory Support, Harefield Hospital NHS Foundation Trust, Harefield, United Kingdom. Electronic address: d.garciasaez@rbht.nhs.uk.
2
Department of Research and Development, TransMedics Inc, Boston, Massachusetts.
3
Department of Cardiothoracic Transplantation and Mechanical Circulatory Support, Harefield Hospital NHS Foundation Trust, Harefield, United Kingdom.

Abstract

BACKGROUND:

Donation after cardiocirculatory death (DCD) has lead to an increase in organ availability. However, because of medical, logistic, and ethical issues, the use of hearts from DCD donors for transplantation is not generally considered to be feasible. In this study, we investigated the feasibility of ex vivo resuscitation and assessment of the porcine heart after circulatory death using the organ care system (OCS).

METHODS:

Cardiocirculatory death was induced in five pigs by cessation of mechanical ventilation. No heparin was administered. The agonal time (AT) was calculated as the time between a reduction of blood pressure <50 mm Hg or a fall in saturation beneath 70% and the cessation of electrical activity. After a further 15 min of warm ischemia, hearts were procured and implanted into the OCS, mimicking the actual clinical scenario for other organs. Thus, procured grafts were assessed ex vivo over a period of 4 h.

RESULTS:

Four hearts were successfully resuscitated on the system (AT 8, 15, 20, and 34 min) Three grafts had excellent visual contractility and lactate trends and were considered to be transplantable. One graft (AT 34 min) had an increased lactate and abnormal contractility being unsuitable for transplantation. One heart with 48-min AT could not be resuscitated.

CONCLUSIONS:

Our data show that hearts from nonheparinized DCD porcine donors can be successfully resuscitated using the OCS in a scenario, which closely simulates clinical conditions.

KEYWORDS:

Cardiocirculatory death; Ex vivo preservation; Heart transplantation

PMID:
25617972
DOI:
10.1016/j.jss.2014.12.039
[Indexed for MEDLINE]

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