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Respir Res. 2015 Jan 24;16:5. doi: 10.1186/s12931-014-0163-5.

Anti-inflammatory deficiencies in neutrophilic asthma: reduced galectin-3 and IL-1RA/IL-1β.

Gao P1,2,3, Gibson PG4,5,6, Baines KJ7,8, Yang IA9,10, Upham JW11,12, Reynolds PN13,14, Hodge S15,16, James AL17,18, Jenkins C19, Peters MJ20,21, Zhang J22, Simpson JL23,24.

Author information

1
Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, China. gaopeng1234@sina.com.
2
Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia. gaopeng1234@sina.com.
3
Priority Research Centre for Asthma and Respiratory Disease, The University of Newcastle, Callaghan, NSW, Australia. gaopeng1234@sina.com.
4
Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia. peter.gibson@hnehealth.nsw.gov.au.
5
Priority Research Centre for Asthma and Respiratory Disease, The University of Newcastle, Callaghan, NSW, Australia. peter.gibson@hnehealth.nsw.gov.au.
6
Woolcock Institute of Medical Research, Glebe, NSW, Australia. peter.gibson@hnehealth.nsw.gov.au.
7
Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia. katherine.baines@newcastle.edu.au.
8
Priority Research Centre for Asthma and Respiratory Disease, The University of Newcastle, Callaghan, NSW, Australia. katherine.baines@newcastle.edu.au.
9
School of Medicine, The University of Queensland, Brisbane, QLD, Australia. YangI@health.qld.gov.au.
10
Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia. YangI@health.qld.gov.au.
11
School of Medicine, The University of Queensland, Brisbane, QLD, Australia. john.upham@uq.edu.au.
12
Department of Respiratory Medicine, Princess Alexandra Hospital, Brisbane, QLD, Australia. john.upham@uq.edu.au.
13
Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, SA, Australia. paul.reynolds@health.sa.gov.au.
14
Lung Research Laboratory, Hanson Institute, Adelaide, SA, Australia. paul.reynolds@health.sa.gov.au.
15
Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, SA, Australia. sandra.hodge@health.sa.gov.au.
16
Lung Research Laboratory, Hanson Institute, Adelaide, SA, Australia. sandra.hodge@health.sa.gov.au.
17
Department of Pulmonary Physiology and Sleep Medicine, Sir Charles Gairdner Hospital, Perth, WA, Australia. alj@westnet.com.au.
18
School of Medicine and Pharmacology, The University of Western Australia, Perth, WA, Australia. alj@westnet.com.au.
19
Respiratory Trials, The George Institute for Global Health, Sydney, NSW, Australia. christine.jenkins@sydney.edu.au.
20
Australian School of Advanced Medicine, Macquarie University, Sydney, NSW, Australia. Matthew.Peters@sswahs.nsw.gov.au.
21
Department of Thoracic Medicine, Concord General Hospital, Concord, NSW, Australia. Matthew.Peters@sswahs.nsw.gov.au.
22
Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, China. doctorzhangj@sina.com.
23
Department of Respiratory and Sleep Medicine, Hunter Medical Research Institute, New Lambton Heights, NSW, Australia. jodie.simpson@newcastle.edu.au.
24
Priority Research Centre for Asthma and Respiratory Disease, The University of Newcastle, Callaghan, NSW, Australia. jodie.simpson@newcastle.edu.au.

Abstract

BACKGROUND:

Galectin-3 (gal-3), a member of the β-galactoside-binding animal lectins, is involved in the recruitment, activation and removal of neutrophils. Neutrophilic asthma is characterized by a persistent elevation of airway neutrophils and impaired efferocytosis. We hypothesized that sputum gal-3 would be reduced in neutrophilic asthma and the expression of gal-3 would be associated with other markers of neutrophilic inflammation.

METHODS:

Adults with asthma (n = 80) underwent a sputum induction following clinical assessment and blood collection. Sputum was dispersed for a differential cell count and ELISA assessment of gal-3, gal-3 binding protein (BP), interleukin (IL)-1β, IL-1 receptor antagonist (RA), IL-8 and IL-6. Gal-3 and gal-3BP immunoreactivity were assessed in mixed sputum cells.

RESULTS:

Sputum gal-3 (median, (q1,q3)) was significantly reduced in neutrophilic asthma (183 ng/mL (91,287)) compared with eosinophilic (293 ng/mL (188,471), p = 0.021) and paucigranulocytic asthma (399 ng/mL (213,514), p = 0.004). The gal-3/gal-3BP ratio and IL-1RA/IL-1β ratio were significantly reduced, while gal-3BP and IL-1β were significantly elevated in neutrophilic asthma compared with eosinophilic and paucigranulocytic asthma.

CONCLUSION:

Patients with neutrophilic asthma have impairment in anti-inflammatory ratio of gal-3/gal-3BP and IL-1RA/IL-1β which provides a further framework for exploration into pathologic mechanisms of asthma phenotypes.

PMID:
25616863
PMCID:
PMC4314745
DOI:
10.1186/s12931-014-0163-5
[Indexed for MEDLINE]
Free PMC Article

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