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Toxicol Ind Health. 2016 Aug;32(8):1486-1494. Epub 2015 Jan 22.

Protective effects of grape seed extract on cadmium-induced testicular damage, apoptosis, and endothelial nitric oxide synthases expression in rats.

Author information

1
Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey drmfatihsonmez@hotmail.com.
2
Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.

Abstract

This study aims to evaluate the protective effect of grape seed proanthocyanidin extract (GSPE) on cadmium (Cd)-induced testicular apoptosis, endothelial nitric oxide synthases (eNOS) expression, and toxicity in rats. A total of 24 male Wistar rats were divided into four groups, namely, control, Cd (2.5 mg/kg), Cd + GSPE (100 mg/kg/day), and GSPE. Spermatogenesis and mean seminiferous tubule diameter were significantly decreased in the Cd groups. Furthermore, the GSPE-treated animals showed an improved histological appearance in the Cd group. The immunoreactivity of eNOS and the number of apoptotic cells were increased in Cd group. Our data indicate a significant reduction of terminal deoxynucleotide transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling staining and a decrease in the expression of eNOS in the testes tissue of the Cd group treated with GSPE therapy. Therefore, our results suggest that GSPE acts as a potent protective agent against Cd-induced testicular toxicity in rats.

KEYWORDS:

Cadmium; apoptosis; eNOS; grape seed proanthocyanidin extract; testis

PMID:
25614582
DOI:
10.1177/0748233714566874
[Indexed for MEDLINE]

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