Format

Send to

Choose Destination
J Neurosci Methods. 2015 Aug 30;252:75-9. doi: 10.1016/j.jneumeth.2015.01.018. Epub 2015 Jan 19.

Simultaneous glutamate recordings in the frontal cortex network with multisite biomorphic microelectrodes: New tools for ADHD research.

Author information

1
Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Center for Microelectrode Technology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Parkinson's Disease Translational Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA.
2
Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Psychiatry, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Neurology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Neurosurgery, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Center for Microelectrode Technology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Parkinson's Disease Translational Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA.
3
Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Pediatrics, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Psychiatry, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Center for Microelectrode Technology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Parkinson's Disease Translational Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA. Electronic address: pglas0@uky.edu.

Abstract

BACKGROUND:

The aberrant regulation of glutamate has been implicated in numerous psychiatric disorders including drug addiction and attention-deficit/hyperactivity disorder. To understand glutamate signaling and its role in facilitating disease, tools to directly measure glutamate in a complex, neural network are needed.

NEW METHOD:

The development of a ceramic-based, dual-sided, biomorphic microelectrode array with four recording sites on each side to facilitate a more detailed measurement of glutamate in awake, behaving rodents.

RESULTS:

In vitro calibrations of these biosensors showed selective and specific responses to glutamate. In awake rats, these biomorphic electrode arrays enabled the concurrent evaluation of glutamate in a network, the frontal cortex: including the cingulate, prelimbic, infralimbic and dorsal peduncle regions. Regions within the frontal cortex exhibited varying phasic glutamate patterns in awake animals.Comparison with existing method: Existing methodologies to measure glutamate neurotransmission employ single-sided biosensors or biosensors capable of measuring neurochemicals at only one location in space.

CONCLUSIONS:

Multi-site, biomorphic neurochemical biosensors provide a method for simultaneously measuring glutamate in multiple areas of a neural network in the brain.

KEYWORDS:

Biosensors; Frontal cortex; Glutamate; Microelectrode array; Neural network

PMID:
25614383
DOI:
10.1016/j.jneumeth.2015.01.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center