Two-dimensional crystalline arrays of Na,K-ATPase with new subunit interactions induced by cobalt-tetrammine-ATP

E Skriver; A B Maunsbach; H Hebert; G Scheiner-Bobis; W Schoner

doi:10.1016/0889-1605(89)90013-x

Two-dimensional crystalline arrays of Na,K-ATPase with new subunit interactions induced by cobalt-tetrammine-ATP

J Ultrastruct Mol Struct Res. 1989 Dec;102(3):189-95. doi: 10.1016/0889-1605(89)90013-x.

Authors

E Skriver¹, A B Maunsbach, H Hebert, G Scheiner-Bobis, W Schoner

Affiliation

¹ Department of Cell Biology, University of Aarhus, Denmark.

PMID: 2561364
DOI: 10.1016/0889-1605(89)90013-x

Abstract

Purified membrane-bound Na,K-ATPase incubated with cobalt-tetrammine-ATP [Co(NH3)4ATP], which is a stable MgATP complex analog, shows two new types of membrane crystals, a new p21 form and a p4 form. The building blocks of the crystalline arrays correspond to (alpha beta)2 dimers of the enzyme protein suggesting that alpha-alpha interaction may be important in the pumping process.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / analogs & derivatives*
Animals
Cobalt*
Crystallization*
In Vitro Techniques
Membrane Proteins / isolation & purification*
Membrane Proteins / ultrastructure
Microscopy, Electron
Organometallic Compounds*
Sodium-Potassium-Exchanging ATPase / isolation & purification*
Swine

Substances

Membrane Proteins
Organometallic Compounds
Cobalt
tetraamminecobalt(III)ATP
Adenosine Triphosphate
Sodium-Potassium-Exchanging ATPase