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J Neurophysiol. 2015 Apr 1;113(7):2461-70. doi: 10.1152/jn.00286.2014. Epub 2015 Jan 21.

Synchronization of action potentials during low-magnesium-induced bursting.

Author information

1
Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia; and.
2
Departments of Neurology and Neuroscience, University of Virginia School of Medicine, Charlottesville, Virginia jk8t@virginia.edu.

Abstract

The relationship between mono- and polysynaptic strength and action potential synchronization was explored using a reduced external Mg(2+) model. Single and dual whole cell patch-clamp recordings were performed in hippocampal cultures in three concentrations of external Mg(2+). In decreased Mg(2+) medium, the individual cells transitioned to spontaneous bursting behavior. In lowered Mg(2+) media the larger excitatory synaptic events were observed more frequently and fewer transmission failures occurred, suggesting strengthened synaptic transmission. The event synchronization was calculated for the neural action potentials of the cell pairs, and it increased in media where Mg(2+) concentration was lowered. Analysis of surrogate data where bursting was present, but no direct or indirect connections existed between the neurons, showed minimal action potential synchronization. This suggests the synchronization of action potentials is a product of the strengthening synaptic connections within neuronal networks.

KEYWORDS:

action potential; dual whole cell patch-clamp; hippocampal culture; synchronization

PMID:
25609103
PMCID:
PMC4416584
DOI:
10.1152/jn.00286.2014
[Indexed for MEDLINE]
Free PMC Article

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