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Am J Physiol Regul Integr Comp Physiol. 2015 Apr 1;308(7):R576-89. doi: 10.1152/ajpregu.00267.2014. Epub 2015 Jan 21.

Angiopoietin-1 enhances skeletal muscle regeneration in mice.

Author information

1
Meakins-Christie Laboratories, Department of Medicine, McGill University, Montréal, Quebec, Canada; Department of Critical Care, McGill University Health Centre, Royal Victoria Hospital, Montréal, Quebec, Canada;
2
Department of Pharmacology and Cancer Biology, Duke University Medical Center and the Duke University School of Medicine, Durham, North Carolina;
3
Department of Anatomy and Cell Biology, McGill University, Montréal, Quebec, Canada; and.
4
Department of Critical Care, McGill University Health Centre, Royal Victoria Hospital, Montréal, Quebec, Canada;
5
Meakins-Christie Laboratories, Department of Medicine, McGill University, Montréal, Quebec, Canada; Département des sciences de la nature, Collège militaire royal de Saint-Jean, Saint-Jean-sur-Richelieu, Quebec, Canada.
6
Meakins-Christie Laboratories, Department of Medicine, McGill University, Montréal, Quebec, Canada; Department of Critical Care, McGill University Health Centre, Royal Victoria Hospital, Montréal, Quebec, Canada; sabah.hussain@muhc.mcgill.ca.

Abstract

Activation of muscle progenitor cell myogenesis and endothelial cell angiogenesis is critical for the recovery of skeletal muscle from injury. Angiopoietin-1 (Ang-1), a ligand of Tie-2 receptors, enhances angiogenesis and skeletal muscle satellite cell survival; however, its role in skeletal muscle regeneration after injury is unknown. We assessed the effects of Ang-1 on fiber regeneration, myogenesis, and angiogenesis in injured skeletal muscle (tibialis anterior, TA) in mice. We also assessed endogenous Ang-1 levels and localization in intact and injured TA muscles. TA fiber injury was triggered by cardiotoxin injection. Endogenous Ang-1 mRNA levels immediately decreased in response to cardiotoxin then increased during the 2 wk. Ang-1 protein was expressed in satellite cells, both in noninjured and recovering TA muscles. Positive Ang-1 staining was present in blood vessels but not in nerve fibers. Four days after the initiation of injury, injection of adenoviral Ang-1 into injured muscles resulted in significant increases in in situ TA muscle contractility, muscle fiber regeneration, and capillary density. In cultured human skeletal myoblasts, recombinant Ang-1 protein increased survival, proliferation, migration, and differentiation into myotubes. The latter effect was associated with significant upregulation of the expression of the myogenic regulatory factors MyoD and Myogenin and certain genes involved in cell cycle regulation. We conclude that Ang-1 strongly enhances skeletal muscle regeneration in response to fiber injury and that this effect is mediated through induction of the myogenesis program in muscle progenitor cells and the angiogenesis program in endothelial cells.

KEYWORDS:

angiogenesis; angiopoietin-1; injury; myoblasts; myogenesis; regeneration; skeletal muscle

PMID:
25608750
PMCID:
PMC4386004
DOI:
10.1152/ajpregu.00267.2014
[Indexed for MEDLINE]
Free PMC Article

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