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Genome Biol. 2014 Dec 21;15(12):553. doi: 10.1186/s13059-014-0553-5.

Evaluation of de novo transcriptome assemblies from RNA-Seq data.

Abstract

De novo RNA-Seq assembly facilitates the study of transcriptomes for species without sequenced genomes, but it is challenging to select the most accurate assembly in this context. To address this challenge, we developed a model-based score, RSEM-EVAL, for evaluating assemblies when the ground truth is unknown. We show that RSEM-EVAL correctly reflects assembly accuracy, as measured by REF-EVAL, a refined set of ground-truth-based scores that we also developed. Guided by RSEM-EVAL, we assembled the transcriptome of the regenerating axolotl limb; this assembly compares favorably to a previous assembly. A software package implementing our methods, DETONATE, is freely available at http://deweylab.biostat.wisc.edu/detonate.

PMID:
25608678
PMCID:
PMC4298084
DOI:
10.1186/s13059-014-0553-5
[Indexed for MEDLINE]
Free PMC Article

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