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J Biol Methods. 2014;1(2). pii: e10.

Advanced methods of microscope control using μManager software.

Author information

1
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
2
Luminous Point, LLC, 626 Prentiss St, San Francisco, CA 94110, USA.
3
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA ; Department of Pathology, University of California, San Francisco, 505 Parnassus Ave, San Francisco, CA 94143 USA.
4
Howard Hughes Medical Institute, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA ; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.

Abstract

μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.

KEYWORDS:

Micro-Manager; high-content screening; high-speed imaging; localization microscopy; open-source microscopy

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