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J Agric Food Chem. 2015 Feb 11;63(5):1505-13. doi: 10.1021/jf505014n. Epub 2015 Jan 29.

Direct and indirect antioxidant activity of polyphenol- and isothiocyanate-enriched fractions from Moringa oleifera.

Author information

1
Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey , 59 Dudley Road, New Brunswick, New Jersey 08901, United States.

Abstract

Moringa oleifera Lam. is a fast-growing, tropical tree with various edible parts used as nutritious food and traditional medicine. This study describes an efficient preparatory strategy to extract and fractionate moringa leaves by fast centrifugal partition chromatography (FCPC) to produce polyphenol and isothiocyanate (ITC) rich fractions. Characterization and further purification of these fractions showed that moringa polyphenols were potent direct antioxidants assayed by oxygen radical absorbance capacity (ORAC), whereas moringa ITCs were effective indirect antioxidants assayed by induction of NAD(P)H quinone oxidoreductase 1 (NQO1) activity in Hepa1c1c7 cells. In addition, purified 4-[(α-l-rhamnosyloxy)benzyl]isothiocyanate and 4-[(4'-O-acetyl-α-l-rhamnosyloxy)benzyl]isothiocyanate were further evaluated for their ORAC and NQO1 inducer potency in comparison with sulforaphane (SF). Both ITCs were as potent as SF in inducing NQO1 activity. These findings suggest that moringa leaves contain a potent mixture of direct and indirect antioxidants that can explain its various health-promoting effects.

KEYWORDS:

4-[(4′-O-acetyl-α-l-rhamnosyloxy)benzyl]isothiocyanate; 4-[(α-l-rhamnosyloxy)benzyl]isothiocyanate; FCPC; Moringa oleifera; NQO1; antioxidants; isothiocyanates; polyphenols

PMID:
25605589
PMCID:
PMC4798254
DOI:
10.1021/jf505014n
[Indexed for MEDLINE]
Free PMC Article

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